Main Article Content
OBJECTIVE: Ameloblastoma in Nigerians presents as a locally aggressive lesion, requiring mutilating jaw surgery. The aetiology is unknown. The prevalence of Human Papilloma Virus (HPV) in ameloblastoma in scientific literature has been inconsistent. Strong association of HPV virus with oropharyngeal and cervical carcinoma has been reported. A high incidence of HPV reported in cervical smears of Nigerian females suggests risk of transmission through oral sex and kissing. In order to contribute to the possible implication of HPV in the aetiology of ameloblastoma, this study aimed to identify and determine the prevalence of HPV in clinico-histologic types of ameloblastoma in Nigerians.
METHODS: Sixty eight samples (35 formalin fixed tissues and 33 formalin fixed paraffin embedded tissues) were selected from a total of 193 cases of ameloblastoma seen during a 10-year period. Haematoxylin and Eosin sections confirmed histological diagnosis in each case. DNA was extracted from each of the 68 samples and Polymerase Chain Reaction was used for genotyping HPV DNA using type specific primers to identify HPV types 11, 16, 18, 31, 33, 35.
RESULTS: HPV 35 DNA was detected in 3 out of the 68 samples (4.4%) of ameloblastoma, one from each of desmoplastic ameloblastoma (DA), solid multicystic ameloblastoma (SMA) and unicystic ameloblastoma (UA). The proportion of DA positive for HPV 35 (25%) was statistically higher than the proportion positive for SMA (1.67%). Chi-square,