IMMUNOHISTOCHEMICAL EXPRESSION OF KI-67 AND P63 IN AMELOBLASTOMA,  ODONTOGENIC KERATOCYST AND DENTIGEROUS CYST : Immunohistochemical expression of ki-67 and p63 in ameloblastoma

C. A. Iyogun; E. V. Orikpete; O. G.  Omitola

Authors

Dr. C A Iyogun Department of Oral Pathology & Biology Faculty of Dentistry, College of Health Sciences University of Port Harcourt, Port Harcourt, Rivers State, Nigeria. Author
Dr. E V Orikpete Department of Oral Pathology & Biology Faculty of Dentistry, College of Health Sciences University of Port Harcourt, Port Harcourt, Rivers State, Nigeria. Author
Prof. O. G. Omitola Department of Oral Pathology & Biology Faculty of Dentistry, College of Health Sciences University of Port Harcourt, Port Harcourt, Rivers State, Nigeria. Author

Keywords:

Immunohistochemical, Ki-67, P63, Ameloblastoma, Odontogenic keratocyst, Dentigerous cyst

Abstract

OBJECTIVE: The aim of this study was to evaluate the
immunohistochemical expressions of Ki-67 and P63 in the epithelial
component of ameloblastoma, OKC and DC, and to determine if
there is any correlation between the expressions of Ki-67 and P63 in
these lesions.
METHODS: A total of 10 ameloblastoma, 4 odontogenic keratocyst
and 9 dentigerous cyst were utilized. Archival tissue blocks were
retrieved and processed for immunohistochemistry using the Avidin
Biotin immunoperoxidase method. The primary antibody utilized
was mouse monoclonal anti-Ki67 and anti-P63 (Novocastra®, 1:100
dilution). Visualization was achieved using diaminobenzidine
(DAB) solution. Stained sections were counterstained using
Haematoxylin, dehydrated using ascending grades of alcohol,
cleared in xylene and cover-slipped using DPX as mountant. They
were then viewed under a microscope for colour reaction.
Data was entered into a spreadsheet and analyzed using Kruskal
Wallis test. Correlation between Ki-67 and P63 was done using
Spearman’s rank correlation coefficient test. P-values ≤ 0.05 were
considered statistically significant. All statistical analysis was done
using IBM SPSS version 23.

RESULTS: Immunohistochemical expression of Ki-67was seen in
100% of OKCs, 70% of ameloblastomas and 44.4% of dentigerous
cysts. For P63, there was positive immunohistochemical expression
in 50% of ameloblastomas, 25% of odontogenic keratocysts and in
11.1% of dentigerous cysts. However, the differences in expression
of these two markers in these lesions was not statistically significant.
Spearman’s rank correlation coefficient test showed that there was
no statistically significant correlation between the expression of Ki
67 and p63 in ameloblastoma (σ = 0.541, P = 0.106), odontogenic
keratocyst (σ = -0.333, P = 0.667) and dentigerous cyst (σ = 0.530,
P = 0.142).
CONCLUSION: The pattern of expression of Ki-67 and P63 by
ameloblastoma, odontogenic keratocyst and dentigerous cyst appears
to follow the clinical aggressiveness of the lesions, with no
correlation of the expression of these two markers.

IMMUNOHISTOCHEMICAL EXPRESSION OF KI-67 AND P63 IN AMELOBLASTOMA, ODONTOGENIC KERATOCYST AND DENTIGEROUS CYST